Download Citation on ResearchGate | Antibacterial activity of Peganum harmala | Successive petroleum ether, chloroform and methanol extracts of Peganum. Successive petroleum ether, chloroform and methanol extracts of Peganum harmala seeds were tested (in vitro) for their antibacterial activity. examine the antibacterial activities of Peganum harmala extracts on the Key words: Antimicrobial effect, Biofilm, Peganum harmala, Pathogenic bacteria.

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Bergey’s manual of systematic bacteriology. Find articles by Shima Dabiri. Author information Article notes Copyright and License information Disclaimer.

Tested against Gram negative bacteria the obtained results were inconsistent. Streptococcus mutans, caries and simulation models. National Committee for Clinical Laboratory Standards; Based on the results from pH treatment Figure 7 Fig.

Some spots are more intensive in one extract comparing with other extract. Ehsanpour AA, Saadat E. In general, combination therapy such as one resulted from synergism between antibiotic and plant extract can be used to as new choice for treatment of infections acgivity by MDR pathogens, to expand antibacterial spectrum, to prevent emergence of MDR pathogens and to minimize the possible toxic effects Aiyegoro et al.

Hengameh Khosropanah 2 Dept. TLC plate visualized at nm B: Antimicrobial properties of Teucrium polium against some clinical pathogens. The absorbance was measured, using a microplate reader at nm.

Antimicrobial activity and phytochemical analysis of Polygonum aviculare L. Asian Pac J Trop Med. Meanwhile, the seed and root extract in combination with carbenicillin showed a relatively weak synergistic activity against MRSA.

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This study investigated the effect of P. Among the evaluated different parts of P. After 3 days, monolayer of Vero cells was formed and washed with PBS. Phytochemical studies of the antibacteerial from Peganum harmala. Study on the effect of Peganum harmala extract on experimental skin wound healing in rat: Thin Layer Chromatography can justify this difference, because the TLC results for seed and root extract showed that these extracts contain several activit with different density or even some bands exist in one extract that we have not been able to find in another one.

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All data were expressed as descriptive analysis mean and standard deviation. Different parts of P. Also, it has been reported that this plant had antibacterial, antifungal and antiviral effects Shonoudam et al.

Unlike the results that are shown in Figures 1 Fig. Another study investigated the sensitivity of K. The reason of the difference between antibacterial effect of these two extracts against Gram positive and negative bacteria was justified using TLC, because of the difference in nature and content of active phytochemicals existing in these extract. Peganum harmala can minimize Escherichia coli infection in antibactdrial, but long-term feeding may induce side effects.

Antimicrobial Activity of Peganum Harmala L. on Streptococcus mutans Compared to % Chlorhexidine

Results of pH activoty treatment of P. Antibacterial activity of root extract against most of the tested Gram positive bacteria was better than seed extract.


Pharmacological effects of Peganum harmala seeds extract on isolated rat uterus. Based on the obtained results, the root extract has a better antibacterial activity against Gram positive bacteria except L.

The least concentration that inhibited colony forming of S. To determine the synergistic effect of the most effective parts of P. The flower, antibacrerial and stem extracts of P. It has bacteriostatic and bactericidal effects with suitable durability in oral cavity.

Further antimicrobial and cytotoxicity studies on animals or human subjects are needed to obtain more harmalla and applicable results. In vitro assay for the anti-brucella activity of medicinal plants against tetracycline-resistant Br. TLC analysis for P. Antibacterial activity of the actjvity extract against tested Bacillus species and S. This process was repeated for three times.

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Antibacterial assay procedure At first, a total of 0. Active extract was fractioned using Thin Layer Chromatography; also their synergism activity in combination with synthetic antibiotic was evaluated.

Absorbance values that were lower than the controls indicated reduction in cell growth. Int J Bio Pharm Res. Antimicrobial effects of Thai medicinal plants against acne-inducing bacteria.