Krameria lappacea (Rhatany or Ratanhia, Krameriaceae) is an endangered, overexploited medicinal plant from the semi-deserts of Andean. Description and images of Krameria lappacea (), a native Chilean plant, provided by the supplier of native exotic Chilean seeds and organizer of plant watching. Krameria lappacea (Rhatany or Ratanhia, Krameriaceae) is an endangered, overexploited medicinal plant from the semi-deserts of Andean South America, and.
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The authors thank Ernst P. Integrated Taxonomic Information System.
Optimal results were obtained by applying the tincture samples directly on top of a polyamide column followed by elution with methanol. Calibration and validation Individual stock solutions of standard compounds were prepared in methanol.
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The composition of the mobile phase consisted of lapoacea. HPLC—MS experiments in positive and negative electrospray ionization mode confirmed identity and peak purity of analytes. Method validation Suitability of the developed method for the quantification of the major lignan derivatives in Ratanhiae radix and Tinctura Ratanhiae can be deduced from several analytical parameters.
Journal of Pharmaceutical and Biomedical Analysis. Selected species [ edit ] Krameria argentea Mart.
Krameria lappacea – Wikiwand
Proanthocyanidins from Lappzcea triandra root. Method development The major problem in the development of a HPLC method for the simultaneous detection of all active lignan derivatives was the structural similarity of the analytes.
One prominent constituent of the investigated extracts 3-formyl 4-hydroxyphenyl E -propenylbenzofuran; compound 7an additional lignan derivative, was isolated in course of the present study.
The content of the lignan derivatives in the tincture samples 2—4 was very consistent with the highest variation for compound 7 0. Standards Compounds krameriaa — 6and 8 — 12 were isolated and their structures elucidated in a previous study .
Attempts to improve the resolution with the aid of additives, such as tetrahydrofuran, 1- and 2-propanol or tert-butylmethyl ether, or a variation of temperature showed no effect.
Repeatability of the method was assured by relative standard deviations of multiple injections. Kottas-Heldenberg and Sohn Vienna, Austria. None of the compounds 1 — 12 could be quantified since their content was below the LOQ and even below the LOD, indicating an exhaustive extraction.
This article has been cited by other articles in PMC. Rhatany is also the name given to krameria roota botanical remedy lappacda of the dried root of para rhatany Krameria argentea or Peruvian rhatany Krameria lappacea.
Krameria lappacea | Andres Puiggros V. | Flickr
In the case of root samples, different solvents methanol, acetone, DCM and mixtures thereof and extraction procedures sonication, ASE extraction were evaluated. Introduction Krameria lappacea Dombey Burdet et Simpson; syn.
Several mixtures thereof Isolation and identification of standards Compounds 1 — 6 and 8 — 12 were obtained and identified in a previous study as: HPLC sample preparation Extract preparation. The methanol insoluble part In order to fit the calibration range the obtained samples were assayed directly for quantification of compounds 1 — 79 — 10 Root preparations of Krameria lappacea Dombey Burdet et Lappaces are traditionally used against oropharyngeal inflammation.
In the course of the present work compound 7, one additional prominent peak, was isolated out of the roots of K. These pharmacological findings make a quality assessment of Ratanhiae radix and its preparations with an adequate analytical method with focus on the class of lignan derivatives, necessary. The drug was introduced into Kramerria medicine over years ago as a remedy against stomach aches, diarrhoea, menstrual problems, nose bleeds and oropharyngeal inflammation [2,3].